AKT3 Mouse Monoclonal Antibody [Clone ID: 9A5.H9.G7]

CAT#: TA398355

AKT3 Western Chemiluminescent Blotting Kit

Product Images

Immunofluorescence of Mouse monoclonal anti-AKT3 antibody. Cell Type: A431 cells. Fixation: 4% paraformaldehyde 10 min. Permeablization: 0.5% Triton X 30 min. Primary Ab: (p/n 200-301-J36) at 1:250 for 72 hours 4°C. Secondary Ab: (p/n 610-142-121) at 1:1000 overnight 4°C.

Specifications

Product Data

• Clone Name: 9A5.H9.G7
• Applications: WB
• Recommended Dilution: WB: User Optimized
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Specificity: The kit is designed to detect both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT1 and AKT2 has not been determined. This kit contains sufficient substrate for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: See kit insert for complete instructions.
• Stability: See kit insert for complete instructions.
• Gene Name: AKT serine/threonine kinase 3
• Database Link:
  Entrez Gene 10000 Human
  UniProt ID Q9Y243
• Background: Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for AKT3 combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of native and/or recombinant human AKT3 protein. AKT3 Western Blotting kit allows for the detection of endogenous protein levels of human AKT3 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT3. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT3 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT3 Western Blotting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.
• Synonyms: AKT 3 western blotting, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, western bloting kit, Chemiluminescent WB, Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit
• Note: Use Rockland Immunochemicals’ Anti-AKT3 Chemiluminescent Kit for Western Blotting for detection of human AKT3 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT3 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.