PRXC, HRPC Mouse Polyclonal Antibody

CAT#: TA396846

Anti-Peroxidase (Mouse) Antibody

Product Images

Western Blot of Mouse anti-Peroxidase (Horseradish) Antibody. Lane 1: Peroxidase (Horseradish). Load: 100ng per lane. Primary antibody: Mouse anti-Peroxidase (Horseradish) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: Fluorescein conjugated mouse secondary antibody at 1:40,000 for 30 min at RT. Block: (MB-070 for 30 minutes at RT. Predicted/Observed size: 44 kDa, 44 kDa for Horseradish Peroxidase.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1:500 - 1:3,000
  IHC: User Optimized
  ELISA: 1:1,000 - 1:5,000
• Host: Mouse
• Clonality: Polyclonal
• Immunogen: Peroxidase [Horseradish]
• Specificity: Anti-Peroxidase (horseradish) (Mouse) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum as well as purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
• Concentration: 1.0mg/mL - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Stability: Expiration date is one (1) year from date of receipt.
• Background: Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Peroxidase (Horseradish) (Mouse) Antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research.
• Synonyms: Mouse anti-peroxidase antibody, mouse anti-HRP, Horseradish peroxidase
• Note: Anti-Peroxidase (Horseradish) (Mouse) antibody has been tested by ELISA and western blot and is suitable for dot blot, immunoprecipitation, conjugation, and most immunological methods requiring high titer and specificity.