CD276 Mouse Antibody [Clone ID: M045]

CAT#: TA389059

Anti-B7-H3/CD276 (Extracellular region) Antibody

Specifications

Product Data

• Clone Name: M045
• Applications: ICC, IP, WB
• Recommended Dilution: WB: 1:1000
  ICC: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Immunogen: Clone (M045) was generated from a recombinant protein that included the extracellular region of human B7-H3 protein.
• Specificity: Clone M045 mouse monoclonal antibody detects a 100-120 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human LNCaP, MCF-7, NCI-H28, A431, and MDA-MB-231 carcinomas. The antibody detects B7-H3 in membranes and cytoplasm in MCF7 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
• Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 100-120
• Database Link:
  UniProt ID Q5ZPR3
• Background: B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 is a membrane protein with an extracellular region that includes two Ig-like V-type domains and two IgG-like C2-type domains, and a short intracellular domain. B7-H3 is a regulatory molecule for immune reactions, such as T cell proliferation and IFN-γ production. In colon cancers, B7-H3 inhibits T-cell cytotoxicity, and blocking B7-H3 function enhances T-cell cytotoxicity toward cancer cells. B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate. In cancer, B7-H3 is expressed in various tumor types including prostate, breast, colon, lung, and gastric cancers. The B7-H3 expression level correlates with tumor growth, invasion, metastasis, malignant stage, and recurrence rate. The inhibition or blockade of B7-H3 function could be an important immunotherapeutic approach for several types of cancer
• Note: Protein G purified tissue culture supernatant.