PRMT5 Rabbit Monoclonal Antibody [Clone ID: RAB-C136]

CAT#: TA386457

Recombinant Anti-PRMT5 (Clone RAB-C136)

Product Images

Flow cytometry using the anti-PRMT5 antibody RAB-C136 (TA386457). HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (TA385792; isotype control, black line) or the rabbit IgG1 version of RAB-C136 (TA386457, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: RAB-C136
• Applications: ChIP, ELISA, IF
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRMT5 protein under non-denaturing conditions.
• Specificity: This antibody recognizes PRMT5 (Protein arginine N-methyltransferase 5). It binds to a folded domain, amino acids 269-637. PRMT5 is a arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA.
  
  This antibody has been used and recommended for the following methods: ELISA; IF; IP-MS; ChIP.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: protein arginine methyltransferase 5
• Database Link:
  Entrez Gene 10419 Human
  UniProt ID O14744
• Synonyms: HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs
• Note: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.