SMARCE1 Rabbit Polyclonal Antibody

CAT#: TA381767

BAF57/SMARCE1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and BAF57/SMARCE1 knockout (KO) 293T cells, using BAF57/SMARCE1 antibody (TA381767) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

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  Western blot analysis of extracts of various cell lines, using BAF57/BAF57/SMARCE1 antibody (TA381767) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of HeLa cells using BAF57/BAF57/SMARCE1 antibody (TA381767).

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  Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug BAF57/BAF57/SMARCE1 antibody (TA381767). Western blot was performed from the immunoprecipitate using BAF57/BAF57/SMARCE1 antibody (TA381767) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:20 - 1:50
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human BAF57/BAF57/SMARCE1 (NP_003070.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 34kDa/37kDa/39kDa/41kDa/42kDa/46kDa
• Gene Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
• Database Link:
  Entrez Gene 6605 Human
  UniProt ID Q969G3
• Background: The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
• Synonyms: BAF57