RAD21 Rabbit Polyclonal Antibody

CAT#: TA380666

Rad21 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Rad21 antibody (TA380666) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Western blot analysis of extracts of C6 cells, using Rad21 antibody (TA380666) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of U2OS cells using Rad21 Rabbit pAb (TA380666) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Rad21 antibody (TA380666). Western blot was performed from the immunoprecipitate using Rad21 antibody (TA380666) at a dilition of 1:1000.

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  Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody (TA380666) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Specifications

Product Data

• Applications: ChIP, ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
  ChIP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human Rad21 .
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: RAD21 cohesin complex component
• Database Link:
  Entrez Gene 5885 Human
  UniProt ID O60216
• Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]
• Synonyms: FLJ25655; FLJ40596; hHR21; HR21; HRAD21; KIAA0078; MCD1; NXP-1; NXP1; SCC1