NUF2 Rabbit Polyclonal Antibody

CAT#: TA371295

NUF2 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: Hela and LO2 cell lysates Primary antibody: TA371295 (NUF2 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 minutes

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA371295 (NUF2 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA371295 (NUF2 Antibody) at dilution 1/60, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Hela and LO2 cell lysates
  IHC: 50-200
  Positive control: Human esophagus cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human NUF2
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 54 kDa
• Gene Name: NUF2, NDC80 kinetochore complex component
• Database Link:
  Entrez Gene 83540 Human
  UniProt ID Q9BZD4
• Background: This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.
• Synonyms: CDCA1; CT106; hNuf2; hNuf2R; hsNuf2; NUF2R