Sprouty 4 (SPRY4) Rabbit Polyclonal Antibody

CAT#: TA319359

Rabbit polyclonal Sprouty-4 antibody

Product Images

Western Blot of Rabbit anti-Sprouty-4 antibody. Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 ug per lane. Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: a doublet band ~35 kDa corresponding to human Sprouty-4. Other band (s): none.

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  Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5 ug/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.

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  Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:3,000 - 1:12,000, WB: 1:100 - 1:1,000, IHC: 1 ug/ml to 10 ug/ml
• Reactivities: Human, Chimpanzee
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 306-322 of Human Sprouty-4 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sprouty RTK signaling antagonist 4
• Database Link:
  NP_112226
  Entrez Gene 81848 Human
  UniProt ID Q9C004
• Synonyms: HH17
• Note: Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.

Reference Data

• Protein Pathways: Jak-STAT signaling pathway