Cullin 3 (CUL3) Rabbit Polyclonal Antibody

CAT#: TA319171

Rabbit polyclonal anti-Cul3 antibody

Product Images

Anti-CUL3 antibody was diluted 1:500 to detect CUL3 in human breast tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:1,000, IHC: User Optimized
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1-12 of Human Cul3 (N-terminus) coupled to KLH.
• Formulation: None
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cullin 3
• Database Link:
  NP_001244126
  Entrez Gene 8452 Human
  UniProt ID Q13618
• Synonyms: CUL-3; PHA2E
• Note: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis