UBE4B Rabbit Polyclonal Antibody

CAT#: TA308624

Rabbit polyclonal antibody to UBE4B (ubiquitination factor E4B (UFD2 homolog, yeast))

Product Images

Sample (30 ug of whole cell lysate). A: Hep G2. 5% SDS PAGE. TA308624 diluted at 1:10000

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  Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using UBE4B (TA308624) antibody at 1:500 dilution.

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  Immunofluorescence analysis of methanol-fixed Hep3B, using UBE4B (TA308624) antibody at 1:500 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:5000-1:20000
• Reactivities: Human (Predicted: Bovine)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 869 and 1179 of UBE4B (Uniprot ID#O95155)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 133 kDa
• Gene Name: ubiquitination factor E4B
• Database Link:
  NP_001099032
  Entrez Gene 10277 Human
  UniProt ID O95155
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: E4; HDNB1; UBOX3; UFD2; UFD2A
• Note: Seq homology of immunogen across species: Bovine (100%)

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis