Apolipoprotein A V (APOA5) Mouse Monoclonal Antibody [Clone ID: 1G5G9]

CAT#: TA301561

Mouse Monoclonal Antibody against Apo A5 (1G5G9)

Product Images

Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) TA301561 - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

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  Immunocytochemistry/Immunofluorescence: Apolipoprotein A5 Antibody (1G5G9) TA301561 - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Apolipoprotein A5 (1G5G9) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: Apolipoprotein A5 Antibody (1G5G9) TA301561 - Analysis of ApoA5 in HepG2 lysates.

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  Western Blot: Apolipoprotein A5 Antibody (1G5G9) TA301561 - Detection of Apo A5 in HepG2 cell lysate with TA301561.

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  Western Blot: Apolipoprotein A5 Antibody (1G5G9) TA301561 - Analysis using anti- human APOA5 monoclonal antibody against human serum total protein (lane 2). Lane 1 is a MW standard.

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Specifications

Product Data

• Clone Name: 1G5G9
• Applications: ELISA, FC, ICC/IF, IHC, WB
• Recommended Dilution: ELISA: 1:100-1:2000, Western Blot: 1:500-1:2000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Ni-NTA purified full-length recombinant human APOA5 expressed in E. Coli strain BL21 (DE3).
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: apolipoprotein A5
• Database Link:
  NP_443200
  Entrez Gene 116519 Human
  UniProt ID Q6Q788
• Background: Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four-fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor-4 (PPAR-alpha) agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Recently, it was demonstrated that ApoA5 is present in human serum detected by polyclonal antibodies against both the NH2 and COOH termini, although at much lower concentrations than other apolipoproteins.
• Synonyms: APOAV; RAP3

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: PPAR signaling pathway