GAPDH Rabbit Polyclonal Antibody

CAT#: SP2162P

GAPDH rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of whole cell lysate from HeLa human epithelial adenocarcinoma cells probed with Rabbit anti Human GAPDH at 0.5 (A), 1 (B) and 2 (C) ug/ml

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  Immunocytochemical staining of HeLa cells with Rabbit anti Human GAPDH

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 0.5 - 1 µg/ml, detects a band of approximately 37 kDa in HeLa cell lysates.
  Immunocytochemistry.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A 16 amino acid peptide located near human GAPDH carboxy-terminus
• Specificity: This antibody recognises an epitope within the C-terminal region (CT) of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0,02% Sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: glyceraldehyde-3-phosphate dehydrogenase
• Database Link:
  Entrez Gene 2597 Human
  UniProt ID P04406
• Background: GAPDH is a glycolytic enzyme which plays a key role in energy production, and has also been implicated in numerous cellular processes.
  GAPDH is a homotetramer molecule consisting of four 36 kDa subunits, constitutively expressed in most cells and tissues, and is responsible for the generation of energy during carbohydrate metabolism, catalysing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate.
  The role of GAPDH as a multifunctional protein has emerged from many studies, confirming its involvement in critical nuclear pathways, apoptosis, membrane transport and fusion, DNA replication and repair and phosphotransferase activity. Furthermore, the ability of GAPDH to bind with high affinity to ß-amyloid precursor protein (Alzheimer's disease) and selectively with CAG mutated proteins, including huntingtin (Huntingdon's disease) and the androgen receptor (spinobulbar muscular atrophy), has focused many studies towards the role of GAPDH in the pathogenesis of neurodegenerative diseases (1).
• Synonyms: GAPD, CDABP0047