Carbonic Anhydrase II (CA2) Sheep Polyclonal Antibody

CAT#: SP1126P

Carbonic Anhydrase II (CA2) sheep polyclonal antibody, Purified

Specifications

Product Data

• Applications: ID, WB
• Recommended Dilution: Western Blot: 1/1000.
  Immunodiffusion.
• Reactivities: Human
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Purified human carbonic anhydrase II (CAII) prepared from erythrocytes.
• Specificity: Product identity is confirmed by double diffusion vs CAII. No reactivity is seen in immunodiffusion against CAI.
• Formulation: Glycine buffered saline pH7.4 containing 0.09% Sodium Azide, 0.1% EACA, 1mM EDTA and 0.01% Benzamidine
  State: Purified
  State: Liquid purified IgG
• Purification: Ion exchange chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: carbonic anhydrase 2
• Database Link:
  Entrez Gene 760 Human
  UniProt ID P00918
• Background: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20µM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.
• Synonyms: Carbonic anhydrase II, Carbonate dehydratase II, Carbonic anhydrase C, CA2, CA-II, CAC