NTAL (LAT2) Mouse Monoclonal Antibody [Clone ID: NAP-07]
Other products for "LAT2"
Specifications
Product Data | |
Clone Name | NAP-07 |
Applications | FC, IF, IP, WB |
Recommended Dilution | Flow Cytometry: Positive tissue: RAJI Human lymphoma cell line. RAMOS Human lymphoma cell line Immunoprecipitation. Western Blot: 2 µg/ml, incubation 60 min. Positive Control: RAMOS Human lymphoma cell line. Negative Control: HeLa Human cervix carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel. Immunocytochemistry. |
Reactivities | Human, Mouse |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Recombinant cytoplasmic domain (aa 91-244) of human NTAL |
Specificity | The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells. |
Formulation | Phosphate buffered saline (PBS) with 15 mM Sodium Azide, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C. DO NOT FREEZE! |
Stability | Shelf life: one year from despatch. |
Gene Name | linker for activation of T-cells family member 2 |
Database Link | |
Background | NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function. |
Synonyms | LAB, NTAL, WBS15, WBSCR5, HSPC046 |
Reference Data |
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