CD44 Mouse Monoclonal Antibody [Clone ID: MEM-263]

CAT#: SM3024F

CD44 mouse monoclonal antibody, clone MEM-263, FITC

Product Images

Western blot analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL

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  Surface staining of human peripheral blood using anti-CD44 (MEM-263) FITC conjugate.

Specifications

Product Data

• Clone Name: MEM-263
• Applications: FC, WB
• Recommended Dilution: Flow Cytometry analysis of Human blood cells: 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human, Porcine, Canine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: COS-7 cells (African Green Monkey)
• Specificity: This antibody reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
• Formulation: PBS
  Label: FITC
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with Fluorescein isothiocyanate under optimum conditions.
  The reagent is free of unconjugated and adjusted for direct use
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This antibody is photosensitive and should be protected from light.
• Stability: Shelf life: One year from despatch.
• Gene Name: CD44 molecule (Indian blood group)
• Database Link:
  Entrez Gene 960 Human
  UniProt ID P16070
• Background: CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
• Synonyms: LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: ECM-receptor interaction, Hematopoietic cell lineage