CD177 Mouse Monoclonal Antibody [Clone ID: MEM-166]

CAT#: SM2003F

CD177 mouse monoclonal antibody, clone MEM-166, FITC

Product Images

Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD177:FITC (SM2003F)

Specifications

Product Data

• Clone Name: MEM-166
• Applications: FC
• Recommended Dilution: Flow cytometry (use 10 µl of neat - 1/10 diluted antibody to label 10e6 cells in 100 µl).
• Reactivities: Human, Monkey, Rhesus Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognises human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97% of the caucasian population. Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). Antibody reactivity and working conditions may vary between species.
• Formulation: PBS with 0.09% sodium azide as preservative and 1% BSA as stabelizer
  Label: FITC
  State: Liquid, purified immunoglobulin fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Protein G affinity chromatography
• Conjugation: FITC
• Storage: Store the antibody at 4°C for one month or at -20°C for longer. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD177 molecule
• Database Link:
  Entrez Gene 57126 Human
  UniProt ID Q8N6Q3
• Background: Cd177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97% of the caucasian population. Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN).
• Synonyms: NB1, PRV1, HNA-2a, HNA2a