FeLV envelope glycoprotein SU gp70 Mouse Monoclonal Antibody [Clone ID: C11D8]

CAT#: SM1727P

FeLV envelope glycoprotein SU gp70 mouse monoclonal antibody, clone C11D8, Purified

Specifications

Product Data

• Clone Name: C11D8
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry.
  Western Blot.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections.
  Neutralization.
  
  Notes: Removal of the preservative is recommended prior to use in functional assays.
  Recommended Dilutions:
  Western Blot 1/100-1/2000, Immunohistochemistry-Paraffin 1/10-1/500, Immunoprecipitation 1/10-1/500, Flow Cytometry 1/10-1/1000, Immunohistochemistry-Frozen 1/10-1/500, Immunohistochemistry 1/10-1/500.
• Reactivities: Feline Leukemia Virus
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Made to Viral FELINE LEUKAEMIA VIRUS gp70
• Specificity: This antibody recognizes the gp85/gp70 envelope glycoprotein of leukaemia virus. The antibody specifically recognises the epitope defined by amino acids 214-218 of the gp70 sequence (aa MGPNL).
  Some weak cross-reactivity with the sequence MGPLL found in the FeLV p15e membrane protein may also occur. This antibody effectively neutralizes all three FeLV subgroups (A,B and C).
• Formulation: PBS, pH 7.2 containing 0.05% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P06752
• Background: FeLV is a retrovirus (family Oncovirinae) that can cause severe immunosuppression, severe non regenerative anemia, and a variety of neoplasms. The 3 main subtypes (FeLVA, FeLVB and FeLVC) may act independently or together to cause a variety of diseases. The major envelope glycoprotein (gp70) is used to bind to target cells and varies with the FeLV subtype.
• Synonyms: Feline leukemia virus env, gp70, p15E