IGJ (JCHAIN) Mouse Monoclonal Antibody [Clone ID: MC19-9]

CAT#: SM1013P

IGJ (JCHAIN) mouse monoclonal antibody, clone MC19-9, Purified

Specifications

Product Data

• Clone Name: MC19-9
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA (20 µg/ml).
  Immunoprecipitation (20 µg/ml).
  Immunohistochemistry on Frozen Sections (1/200).
  Western blotting (only under Reducing Conditions).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human J Chain.
• Specificity: The antibody reacts with Human J chain as tested by ELISA using purified Human Ig fragments as the solid phase target antigen.
  The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance.
  In Western blotting techniques SM1013P detects J chain in IgM only in reducing conditions.
• Formulation: TRIS buffered Saline containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: joining chain of multimeric IgA and IgM
• Database Link:
  Entrez Gene 3512 Human
  UniProt ID P01591
• Background: Immunoglobulin J chain (IGJ) serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also helps to bind these immunoglobulins to secretory components.
• Synonyms: IGJ, IGCJ