MCP3 (CCL7) Goat Polyclonal Antibody

CAT#: PP1046B1

MCP3 (CCL7) goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human MCP-3 by Direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2–0.4 ng/well of recombinant Human MCP-3.
  Sandwich ELISA: To detect Human MCP-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Human MCP-3 (Cat.-No PP1046P) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MCP-3.
  Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) E.coli derived recombinant Human MCP3 / CCL7 (Cat.-No PA100)
• Specificity: This antibody recognizes Human Macrophage Chemotactic Protein-3 (MCP-3/CCL7).
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: C-C motif chemokine ligand 7
• Database Link:
  Entrez Gene 6354 Human
  UniProt ID P80098
• Background: This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
• Synonyms: Small-inducible cytokine A7, CCL-7, MCP-3, C-C motif chemokine 7, SCYA6, SCYA7, NC28
• Note: Centrifuge vial prior to opening!