VEGF Receptor 1 (FLT1) Mouse Monoclonal Antibody [Clone ID: #EWC]

CAT#: DM3504B

VEGF Receptor 1 (FLT1) mouse monoclonal antibody, clone #EWC (formerly FLTEWC), Biotin

Specifications

Product Data

• Clone Name: #EWC
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA: 1.5–8 µg/ml IgG1.
  Western Blot: 1–10 µg/ml IgG1.
  
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human soluble extracellular Flt-1 (D5) (Cat.-No DA3539X)
• Specificity: The unconjugated monoclonal antibody will detect native and denatured Human VEGFR-1/Flt-1 in ELISA experiments and on the surface of different Human cell types.
  The antibody will also detect Mouse Flt-1 (e.g. natural occurring soluble Mouse Flt-1).
  
  This clone was formerly named FLTEWC.
• Formulation: PBS
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Stabilizer: 50x BSA
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Protein G Chromatography
• Conjugation: Biotin
• Storage: Prior to reconstitution store at 2-8°C for one month or at -20°C for 6 month.
  Following reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: fms related tyrosine kinase 1
• Database Link:
  Entrez Gene 2321 Human
  UniProt ID P17948
• Background: Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 (sVEGFR-1) is the naturally occurring form and was cloned from total RNA of human umbilical vein endothelial cells.
  The mature rh-sVEGFR-1 is a glycosylated monomeric protein with a mass of approximately 96kDa. The soluble receptor consists of the first 6 extracellular domains containing the unique 31 amino acids residues at the C-terminus. Endothelial cells express three different vascular VEGF receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells.
  The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly, a naturally occurring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
  The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis binding VEGF with the same affinity as the full-length receptor.
• Synonyms: VEGFR1, FLT1, FLT, FRT, VEGF Receptor 1