Neurofilament (NEFL) (+ Neurofilament H) Mouse Monoclonal Antibody [Clone ID: 2F11]

CAT#: DM080S

Neurofilament (NEFL) (+ Neurofilament H) mouse monoclonal antibody, clone 2F11, Supernatant

Specifications

Product Data

• Clone Name: 2F11
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/20.
  Immunohistochemistry on Frozen Sections: 1/160, preferably in PBS.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
  Pretreatment with TUF is recommended.
  Positive Control: Brain.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Neurofilament from Human brain.
• Specificity:

  This antibody stains the 70 kD and 200 kD polypeptides of Neurofilament.
  Not reactive with small cell lung carcinoma.
  Cross reactivity
  GFAP: : not detectable
  Keratin : not detectable
  Vimentin : not detectable
  Desmin : not detectable

• Formulation: State: Supernatant
  State: Liquid Supernatant
  Preservative: 10 mM Sodium Azide, 1% FCS
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C.

• Stability: Shelf life: one year from despatch.

• Gene Name: neurofilament, light polypeptide
• Database Link:
  Entrez Gene 4747 Human
  UniProt ID P07196
• Background: Neurofilaments are the intermediate filaments of neurons and their processes. They are protein triplets composed of three major subunits of MW 68kDa, 155kDa, and 200kDa. Neurofilaments are expressed in tumors of neural origin, or displaying neuronal differentiation, such as neuroblastoma, medulloblastoma, and retinoblastoma.
• Synonyms: NF-L,NEFL, NF68, 68 kDa neurofilament protein
• Note: Mol. Wt. of Antigen: 68kDa and 200kDa (phosphorylated forms of these subunits)