Interleukin-4 / IL4 Rat Monoclonal Antibody [Clone ID: 11B4]
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Specifications
Product Data | |
Clone Name | 11B4 |
Applications | ELISA, IP, Neutralize |
Recommended Dilution | ELISA, IP, Neutralization of IL4 bioactivity. |
Reactivities | Human |
Host | Rat |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Human IL4-transfected Cos7 cells. |
Specificity | Human interleukin-4. |
Formulation | Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol. State: Purified |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage | -20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing. |
Database Link | |
Background | Interleukin-4 (IL-4) is a monomeric 20 kDa predominantly produced by activated T lymphocytes of the Th2 phenotype, by mast cells and by some antigen-presenting cells. Together with IL-2, IL-4 is an autocrine growth factor for activated T cells and for cytotoxic T cell precursors. IL-4 is a major factor involved in the activation of resting B lymphocytes. Together with IL-13, IL-4 induces isotype switch towards IgE and IgG1 isotypes. 11B4, was produced from a rat immunized with purified human IL4-transfected-COS7 cells. It recognized both E. coli-expressed and mammalian cell-expressed (COS7 and L cell), rhuIL-4 in solution (immunoprecipitation), as well as on solid phase (indirect ELISA and dot-blotting). The 11B4 antibody inhibited IL-4 bioactivity at an IC50 which was 25-50-fold in molar excess of factor. The 11B4 antibody was used to develop an immunoenzymatic assay capable of detecting concentrations less than 100 pg/ml. (Chrétien I et al, 1989, J. Immunol. Methods, 1178 (1) : 67-81; Ramanathan L.et al, 1993, Biochemistry, 32 (14) :3549-56). |
Synonyms | IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra |
Reference Data | |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, T cell receptor signaling pathway |
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