Dnmt2 (TRDMT1) Mouse Monoclonal Antibody [Clone ID: OTI8H10]

CAT#: CF811494

Carrier-free (BSA/glycerol-free) TRDMT1 mouse monoclonal antibody,clone OTI8H10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRDMT1 (Cat# RC208363, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRDMT1 (Cat# TA811494)(1:2000). Positive lysates LY401401 (100ug) and LC401401 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from mouse liver tissue lysate by using anti-TRDMT1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI8H10
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 84-293 of human TRDMT1 (NP_004403) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.4 kDa
• Gene Name: tRNA aspartic acid methyltransferase 1
• Database Link:
  NP_004403
  Entrez Gene 13434 MouseEntrez Gene 1787 Human
  UniProt ID O14717
• Background: This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]
• Synonyms: DMNT2; DNMT2; MHSAIIP; PUMET; RNMT1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways