NOR1 (NR4A3) Mouse Monoclonal Antibody [Clone ID: OTI2B11]

CAT#: CF804854

Carrier-free (glycerol/BSA-free) NR4A3 mouse monoclonal antibody, clone OTI2B11 (formerly 2B11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR4A3 (RC218250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR4A3. Positive lysates LY429326 (100ug) and LC429326 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804854)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804854)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804854)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804854)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NR4A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804854)

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Specifications

Product Data

• Clone Name: OTI2B11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 332-626 of human NR4A3 (NP_008912) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68 kDa
• Gene Name: nuclear receptor subfamily 4 group A member 3
• Database Link:
  NP_008912
  Entrez Gene 18124 MouseEntrez Gene 58853 RatEntrez Gene 8013 Human
  UniProt ID Q92570
• Background: This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcriptional activator. The protein can efficiently bind the NGFI-B Response Element (NBRE). Three different versions of extraskeletal myxoid chondrosarcomas (EMCs) are the result of reciprocal translocations between this gene and other genes. The translocation breakpoints are associated with Nuclear Receptor Subfamily 4, Group A, Member 3 (on chromosome 9) and either Ewing Sarcome Breakpoint Region 1 (on chromosome 22), RNA Polymerase II, TATA Box-Binding Protein-Associated Factor, 68-KD (on chromosome 17), or Transcription factor 12 (on chromosome 15). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
• Synonyms: CHN; CSMF; MINOR; NOR1

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors