RARRES1 Mouse Monoclonal Antibody [Clone ID: OTI1B3]

CAT#: CF506088

Carrier-free (BSA/glycerol-free) RARRES1 mouse monoclonal antibody, clone OTI1B3 (formerly 1B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RARRES1 (RC205143, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RARRES1. Positive lysates LY404197 (100ug) and LC404197 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RARRES1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Anti-RARRES1 mouse monoclonal antibody (TA506088) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RARRES1 (RC205143).

Specifications

Product Data

• Clone Name: OTI1B3
• Applications: IF, WB
• Recommended Dilution: WB 1:200~4000, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RARRES1(NP_996846) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.1 kDa
• Gene Name: retinoic acid receptor responder 1
• Database Link:
  NP_996846
  Entrez Gene 109222 MouseEntrez Gene 310486 RatEntrez Gene 612298 DogEntrez Gene 703781 MonkeyEntrez Gene 5918 Human
  UniProt ID P49788
• Background: This gene was identified as a retinoid acid (RA) receptor-responsive gene. It encodes a type 1 membrane protein. The expression of this gene is upregulated by tazarotene as well as by retinoic acid receptors. The expression of this gene is found to be downregulated in prostate cancer, which is caused by the methylation of its promoter and CpG island. Alternatively spliced transcript variant encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
• Synonyms: LXNL; PERG-1; TIG1

Reference Data

• Protein Families: Druggable Genome