CALCOCO2 Mouse Monoclonal Antibody [Clone ID: OTI4H5]

CAT#: CF501971

Carrier-free (BSA/glycerol-free) CALCOCO2 mouse monoclonal antibody, clone OTI4H5 (formerly 4H5)

Product Images

Anti-CALCOCO2 mouse monoclonal antibody (TA501971) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CALCOCO2 (RC203843).

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CALCOCO2 monoclonal antibody.

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CALCOCO2 (Cat# RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2(Cat# TA501971). Positive lysates LY417045 (100ug) and LC417045 (20ug) can be purchased separately from OriGene.

• 

  HEK293T cells transfected with either RC203843 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CALCOCO2 antibody (TA501971), and then analyzed by flow cytometry.

• 

  Flow cytometric Analysis of Jurkat cells, using anti-CALCOCO2 antibody (TA501971), (Red), compared to a nonspecific negative control antibody, (Blue).

• 

  Flow cytometric Analysis of Hela cells, using anti-CALCOCO2 antibody (TA501971), (Red), compared to a nonspecific negative control antibody, (Blue).

• Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-CALCOCO2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

• Figure from citation: Western Blot of CALCOCO2 (NDP52) protein level by using anti-CALCOCO2 antibody in human HEp-2 cell lysates. View Citation

Show all 8 images

Specifications

Product Data

• Clone Name: OTI4H5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CALCOCO2 (NP_005822) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.1 kDa
• Gene Name: calcium binding and coiled-coil domain 2
• Database Link:
  NP_005822
  Entrez Gene 697836 MonkeyEntrez Gene 10241 Human
  UniProt ID Q13137
• Background: The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: NDP52