Nucleolar Protein xNOPP180 Mouse Monoclonal Antibody [Clone ID: No-114]
CAT#: BM5066
Nucleolar Protein xNOPP180 mouse monoclonal antibody, clone No-114, Supernatant
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Specifications
Product Data | |
Clone Name | No-114 |
Applications | IF, IHC, IP, WB |
Recommended Dilution | Immunoblotting: 1:50. Immunoprecipitation. Immunofluorescence microscopy. Immunohistochemistry on frozen sections (also after fixation with paraformaldehyde): 1:5 - 1:10; 1 h at RT. |
Reactivities | Xenopus |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Nucleolar fraction prepared from Xenopus laevis oocytes |
Specificity | The antibody stains specifically the nucleolus and Cajal bodies in interphase cells. The antigen is a constitutively expressed nucleolar component and a specific marker for the dense fibrillar component of the interphase nucleolus. The protein recognized is a 180 kD polypeptide in Xenopus (xNopp180), homologue to the rat polypeptide (Nopp140). When applied at high concentration to human cells the antibody reacts with a nucleolar component which, however, does not correspond to xNopp180. |
Formulation | State: Supernatant State: Liquid culture supernatant contains 0.09% NaN3 |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! |
Stability | Shelf life: one year from despatch. |
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