hCG beta (CGB3) Mouse Monoclonal Antibody [Clone ID: H6]

CAT#: BM4066

hCG beta (CGB3) mouse monoclonal antibody, clone H6, Purified

Product Images

BM4066 CD163 antibody staining of Paraffin Human Placenta Section (Clone h6)

Specifications

Product Data

• Clone Name: H6
• Applications: ELISA, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: Does not react on routinely processed frozen sections.
  Immunohistochemistry on Paraffin Sections: 2 µg/ml (1/200). Proteinase K pretreatment for antigen retrieval is recommended.
  It works also in ELISA.
  Suggested Positive Control: Human placenta.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This Monoclonal antibody Clone h6 recognizes β-HCG, a well defined oncofetal protein of 36.7 kDa m.w. associated with pregnancy and some cancers. Raised serum levels for HCG are found in teratogenic carcinomas and trophoblastic tumors and occasionally in gastrointestinal, urogenital, bronchial or mammary carcinomas, and in lymphomas.
• Formulation: PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% Thimerosal as a preservative
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.4 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: chorionic gonadotropin beta subunit 3
• Database Link:
  Entrez Gene 1082 Human
  UniProt ID P0DN86
• Background: Human gonadothropin is a glycoprotein of 36.7kDa consisting of two subunits. The α-chain (MW 14.5 kD) is common to three other glycoprotein hormones (TSH, FSH, LH) whereas the β-chain (22.2kD) is more different. The β-chain of HCG and LH (lutrophin) have sequence homology in the first 112 amino acids and differ at the C-terminal end consisting of 24 amino acids.
• Synonyms: Choriogonadotropin subunit beta, CGB, CGB3, beta hCG, hCG-beta
• Note: Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
  The whole procedure is performed at room temperature
  1. Wash in PBS
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer's hemalum.
  
  Protocol with formalin-fixed, paraffin-embedded sections:
  The whole procedure is performed at room temperature
  1. Deparaffinize and rehydrate tissue section
  2. Place slide in a cuvette with 250ml 0.01M citrate buffer, pH 6.0
  3. Heat slide in a microwave oven for 2 x 7min. at 700 Watt
  4. Leave slide in the buffer for 20min for cooling
  5. Wash in distilled water
  6. Block endogenous peroxidase
  7. Wash in PBS
  8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  10. Wash in PBS
  11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
  12. Wash in PBS
  13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  14. Wash in PBS
  15. Counterstain with Mayer's hemalum.