Nucleophosmin (NPM1) Rabbit Polyclonal Antibody

CAT#: AP21057PU-N

Nucleophosmin (NPM1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of NPM antibody (Cat.-No.: AP21057PU-N) in extracts from HeLa cells.

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  Immunohistochemistry (IHC) analyzes of NPM antibody (Cat.-No.: AP21057PU-N) in paraffin-embedded human tonsil tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of NPM protein.
  (region surrounding Lys230)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 33 kDa
• Gene Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Database Link:
  Entrez Gene 18148 MouseEntrez Gene 25498 RatEntrez Gene 4869 Human
  UniProt ID P06748
• Background: This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
• Synonyms: NPM, NPM1, Numatrin

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors