PGK1 (Center) Rabbit Polyclonal Antibody

CAT#: AP13683PU-N

PGK1 (Center) rabbit polyclonal antibody, Purified

Product Images

The anti-PGK1 Pab is used in Western blot to detect PGK1 in mouse stomach tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100 - 1/500.
  Immunohistochemistry 1/50 - 1/100.
  Flow cytometry.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PGK1.
• Specificity: This antibody reacts to PGK1.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: phosphoglycerate kinase 1
• Database Link:
  Entrez Gene 5230 Human
  UniProt ID P00558
• Background: Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.
• Synonyms: PGKA, PRP 2