Raptor (RPTOR) pSer863 Rabbit Polyclonal Antibody

CAT#: AP12910PU-N

Raptor (RPTOR) pSer863 rabbit polyclonal antibody, Aff - Purified

Product Images

Dot blot analysis of anti-raptor-pSer863 Pab (Cat#AP12910PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Specifications

Product Data

• Recommended Dilution: ELISA: 1/1,000.
  Dot Blot: 1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S863 of human Raptor.
• Specificity: This antibody detects Raptor pSer863.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: regulatory associated protein of MTOR complex 1
• Database Link:
  Entrez Gene 57521 Human
  UniProt ID Q8N122
• Background: Raptor participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). It plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. It has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, raptor serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions.
• Synonyms: RPTOR, KIAA1303, Regulatory-associated protein of mTOR, P150 target of rapamycin TOR-scaffold protein
• Note: Molecular weight: 149038 Da