TNIK pSer764 Rabbit Polyclonal Antibody

CAT#: AP12728PU-N

TNIK pSer764 rabbit polyclonal antibody, Aff - Purified

Product Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Dot blot analysis of Phospho-TNIK pSer764 Pab and TNIK-pSer764 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. P-Pab = phosphorylated antibody, NP-Pab = non-phophorylated antibody, P-Pep = phospho-peptide, NP-Pep = non-phospho-peptide.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: ELISA: 1/1,000.
  Immunohistochemistry: 1/50-1/100.
  Dot Blot: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S764 of human TNIK.
• Specificity: This antibody detects TNIK pSer764.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: TRAF2 and NCK interacting kinase
• Database Link:
  Entrez Gene 23043 Human
  UniProt ID Q9UKE5
• Background: TNIK is a stress-activated serine/threonine kinase that may play a role in the response to environmental stress. This protein appears to act upstream of the JUN N-terminal pathway, and may play a role in cytoskeletal regulation.
• Synonyms: KIAA0551
• Note: Molecular weight: 154943 Da