CAMK2B (beta/gamma) Rabbit Polyclonal Antibody

CAT#: AP06726PU-N

CAMK2B (beta/gamma) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CaMKIIβ/? antibody at 1/500 dilution Lane 1:HEK293T whole cell lysate Lane 2:Raw264.7 whole cell lysate Lane 3:Rat colon tissue lysate

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 601-652of Human CaMKIIβ.
• Specificity: This antibody detects endogenous levels of CaMKIIβ/γ protein.
  (region surrounding Arg631)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 62 kDa
• Gene Name: calcium/calmodulin dependent protein kinase II beta
• Database Link:
  Entrez Gene 816 Human
  UniProt ID Q13554
• Background: The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated α, β, γ and δ, which may or may not be coexpressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10 - 40 - fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory threonine residue at position 177.
• Synonyms: CAM2, CAMKB, CaM-kinase II beta chain, CaM kinase II subunit beta, CaMK-II subunit beta, CAMKIIb