Cytokeratin 17 (KRT17) Rabbit Polyclonal Antibody

CAT#: AP06198PU-N

Cytokeratin 17 (KRT17) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Cytokeratin 17 antibody in extracts from PC12 cells.

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  Immunohistochemistry (IHC) analyzes of Keratin 17/36 antibody in paraffin-embedded human breast carcinoma tissue

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human Cytokeratin 17.
• Specificity: This antibody detects endogenous levels of Keratin 17/36 protein.
  (region surrounding Val412)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 50 kDa
• Gene Name: keratin 17
• Database Link:
  Entrez Gene 3872 Human
  UniProt ID Q04695
• Background: Cytokeratin 17 is a member of the Cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Cytokeratin 17 contains 432 amino acids and is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Cytokeratin 17 functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3-sigma to mediate protein synthesis. Mutations in the gene encoding for Cytokeratin 17 lead to depressed protein translation and smaller sized skin keratinocytes, corresponding to decreased Akt/mTOR signaling activity. Cytokeratin 17 may be a useful marker for cervical stem cell identification, squamous cell carcinoma of the larynx, respiratory syncytial virus and transitional cell carcinomas of the human urinary tract.
• Synonyms: Cytokeratin-17, Keratin-17, Keratin 17, KRT17, CK17, K17