EMA (MUC1) Mouse Monoclonal Antibody [Clone ID: VU-2G7]

CAT#: AM32165PU-N

EMA (MUC1) mouse monoclonal antibody, clone VU-2G7, Purified

Specifications

Product Data

• Clone Name: VU-2G7
• Applications: ELISA, IHC
• Recommended Dilution: ELISA.
  Immunohistochemistry on frozen and paraffin sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A Balb/c mouse was immunized with synthetically glycosylated MUC1 60mer tandem repeat NH2-(HGVTSAPDT(GalNAc)RPAPGSTAPPAHG)3-COOH, conjugated to bovine serum albumin.
  Splenocytes were fused with SP2/0 mouse myeloma cells.
• Specificity: This antibody reacts to MUC1.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0,05% sodium azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: mucin 1, cell surface associated
• Database Link:
  Entrez Gene 4582 Human
  UniProt ID P15941
• Background:

  VU-2G7 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of VU-2G7 has not been established with “epitope fingerprinting”.

• Synonyms: MUC-1, PEMT, Episialin, EMA, H23AG, PUM, DF3, CA 15-3