TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: 4H31]

CAT#: AM26299PU-N

TNF alpha (TNF) mouse monoclonal antibody, clone 4H31, Purified

Specifications

Product Data

• Clone Name: 4H31
• Applications: ELISA, FN, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry on frozen sections: The typical starting working dilution is 1:10.
  Flow cytometry: The typical starting working dilution is 1:10.
  Functional assays.
  Immunoassays.
  Immunoprecipitation.
  Western blot: The typical starting working dilution is 1:10.
  Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The antibody reacts with human native and recombinant TNF-alpha as assessed by ELISA. The antibody inhibits the biological activity of human native and recombinant TNF-alpha as determined with L929 cells in a cytotoxicity assay. The antibody cross reacts with rhesus and cynomolgus natural TNF-alpha and lacks crossreactivity with human lymphotoxin.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein G
• Conjugation: Unconjugated
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 7124 Human
  UniProt ID P01375
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin