BPV-1 Protein E2 (208-218) Mouse Monoclonal Antibody [Clone ID: 1H10]

CAT#: AM26102PU-N

BPV-1 Protein E2 (208-218) mouse monoclonal antibody, clone 1H10, Aff - Purified

Product Images

Western Blot testing of anti-BPV E2 monoclonal antibody (1E2). Testing recombinant purified BPV E2 protein-. Different BPV E2 dilutions were used for testing. Line 1: 10 ng, Line 2: 5 ng, Line 3: 1 ng, Line 4: 0.5 ng</p>

Specifications

Product Data

• Clone Name: 1H10
• Applications: IF, WB
• Recommended Dilution: Immunofluorescence (1/1000): Dilute antibody to a concentration of 1-2 μg/ml in a diluent containing 1% BSA in PBS. Incubate with diluted antibody for 1 hour at room temperature.
  Western blot (1/1000–1/5000): Dilute to a concentration of 1.0-0.2 μg/ml in a diluent containing 2% non-fat dry milk in TBST (Tris-Buffered Saline, Tween-20). Incubate the membrane with diluted the antibody for 1 hour at room temperature with gentle orbital shaking.
• Reactivities: Bovine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant full length BPV type 1 E2 protein purified from E. coli
• Specificity: This antibody detects Bovine Papillomavirus E2-protein and E10Tag (mapped to aa 208-218).
• Formulation: PBS with 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20 to -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P03122
• Background: The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
• Synonyms: BPV1 Regulatory protein E2