AGR3 Mouse Monoclonal Antibody [Clone ID: AGR3.1]

CAT#: AM26017PU-N

AGR3 mouse monoclonal antibody, clone AGR3.1, Aff - Purified

Product Images

Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.

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  Immunohistochemistry staining of AGR3 with Mouse monoclonal AGR3.1 in Paraffin-Embedded Sections of breast carcinoma.

Specifications

Product Data

• Clone Name: AGR3.1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Western blot: 1 μg/ml.
  Positive Control: T47D breast cancer cell line.
  Negative Control: H1299 lung carcinoma cell line.
  Immunohistochemistry on Paraffin Sections: 1 μg/ml.
  Positive Tissue: breast cancer.
  Immunohistochemistry on Frozen Sections: 1 μg/ml.
  Positive Tissue: breast cancer.
  Immunocytochemistry: 1 μg/ml.
  Positive Control: T47D breast cancer cell line.
  Negative Control: H1299 lung carcinoma cell line.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human AGR3 protein.
• Specificity: This antibody recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa), a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Stabilizer: 0.2% (w/v) high-grade protease free BSA
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: anterior gradient 3, protein disulphide isomerase family member
• Database Link:
  Entrez Gene 155465 Human
  UniProt ID Q8TD06
• Background: AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.
• Synonyms: BCMP11, HAG3, HAG-3