MASPIN (SERPINB5) Mouse Monoclonal Antibody [Clone ID: AT2N6]

CAT#: AM09353PU-N

MASPIN (SERPINB5) mouse monoclonal antibody, clone AT2N6, Purified

Product Images

Cell lysates of HeLa (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: Cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green).

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  Immunofluorescence analysis : Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green).

Specifications

Product Data

• Clone Name: AT2N6
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Immunofluorescence.
  Western blot (1:1,000 - 1:2,000).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Maspin (1-375 aa) purified from E. coli
• Specificity: The antibody recognizes human Serpin B5 (Maspin). Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: serpin family B member 5
• Database Link:
  Entrez Gene 5268 Human
  UniProt ID P36952
• Background: Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but downregulated in the respective carcinomas.
• Synonyms: Serpin B5, PI5, PI-5

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: p53 signaling pathway