MAT2A Mouse Monoclonal Antibody [Clone ID: AT3A2]

CAT#: AM09067PU-N

MAT2A mouse monoclonal antibody, clone AT3A2, Purified

Product Images

Cell lysates of HepG2 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MAT2A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: Cell lysates of HepG2 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MAT2A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Paraffin embedded sections of human colon cancer tissue were incubated with anti-human MAT2A (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

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  Immunohistochemistry: Paraffin embedded sections of human colon cancer tissue were incubated with anti-human MAT2A ( 1 : 50 ) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: AT3A2
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1:1,000 - 1:2,000)).
  Immunohistochemistry on Paraffin embedded tissues (1:50 - 1:100).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human MAT2A (1-395 aa) purified from E. coli
• Specificity: The antibody recognizes human MAT2A. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: methionine adenosyltransferase 2A
• Database Link:
  Entrez Gene 4144 Human
  UniProt ID P31153
• Background: Methionine adenosyltransferase II (MAT II) is a key enzyme in cellular metabolism and catalyzes the formation of S-adenosylmethionine (SAMe) from L-methionine and ATP.
  MAT2A is expressed in extrahepatic tissues. In liver, MAT2A expression associates with growth, dedifferentiation, and cancer.
• Synonyms: S-adenosylmethionine synthetase isoform type-2, AdoMet synthetase 2, AMS2, MATA2

Reference Data

• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism