Rab5 (RAB5A) Mouse Monoclonal Antibody [Clone ID: 3A4]

CAT#: AM09031PU-N

Rab5 (RAB5A) mouse monoclonal antibody, clone 3A4, Purified

Product Images

The extracts of mouse brain (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis on Mouse brain: The extracts of mouse brain (45 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: 3A4
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot (1/500-1/2,000).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human Rab5a (1-215 aa) purified from E. coli
• Specificity: The antibody recognizes Human and Mouse RAB5A. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: RAB5A, member RAS oncogene family
• Database Link:
  Entrez Gene 5868 Human
  UniProt ID P20339
• Background: Rab5a is a member of the RAS oncogene family. Rab5a is a small GTPase localized to the plasma membrane and early endosomes. It is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.
• Synonyms: Rab-5A