Cytokeratin 17 (KRT17) Mouse Monoclonal Antibody [Clone ID: 3B12]

CAT#: AM06354SU-N

Cytokeratin 17 (KRT17) mouse monoclonal antibody, clone 3B12, Ascites

Product Images

Western blot analysis using Cytokeratin 17 antibody Cat.-No AM06354SU-N against Hela (Lane 1), MCF-7 (Lane 2) and A431 (Lane 3) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human lung cancer (A), endometrial carcinoma (B), sublingual gland (C) and esophagus (D) tissues using Cytokeratin 17 antibody Cat.-No AM06354SU-N with DAB staining.

Specifications

Product Data

• Clone Name: 3B12
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of CK17 expressed in E. Coli.
• Specificity: Recognizes Cytokeratin 17
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 49 kDa
• Gene Name: keratin 17
• Database Link:
  Entrez Gene 3872 Human
  UniProt ID Q04695
• Background: CK17, also known as KRT17, it is the type I intermediate filament chain keratin 17. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
• Synonyms: Cytokeratin-17, Keratin-17, Keratin 17, KRT17, CK17, K17