CD18 (ITGB2) Mouse Monoclonal Antibody [Clone ID: 10E12]

CAT#: AM06337SU-N

CD18 (ITGB2) mouse monoclonal antibody, clone 10E12, Ascites

Product Images

Confocal immunofluorescence analysis of HL60 cells using CD18 antibody Cat.-No AM06337SU-N (green). Blue: DRAQ5 fluorescent DNA dye.

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  Confocal immunofluorescence analysis of BCBL-1 cells (left) and L1210 cells (right) using CD18 antibody Cat.-No AM06337SU-N (red). Blue: DRAQ5 fluorescent DNA dye.

Specifications

Product Data

• Clone Name: 10E12
• Applications: ELISA, IF
• Recommended Dilution: ELISA: 1/10000.
  Immunofluorescence: 1/200 - 1/1000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of CD18 expressed in E. Coli.
• Specificity: Recognizes CD18 also known as ITGB2 (integrin beta chain beta 2).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 85 kDa
• Gene Name: integrin subunit beta 2
• Database Link:
  Entrez Gene 3689 Human
  UniProt ID P05107
• Background: CD18, also known as ITGB2 (integrin beta chain beta 2). Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. CD18 is expressed by most leucocytes. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene.
• Synonyms: Integrin beta-2, MFI7