Pam (NM_013626) Mouse Recombinant Protein

CAT#: TP523751

Purified recombinant protein of Mouse peptidylglycine alpha-amidating monooxygenase (Pam), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR223751 representing NM_013626
  Red=Cloning site Green=Tags(s)
  
  MAGRARSRLLLLLGLLALQSSCLAFRSPLSVFKRFKETTRSFSNECLGTTRPITPIDSSDFTLDIRMPGV
  TPKESDTYFCMSMRLPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAW
  ARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSLHLTRVPQPLIAGMYLMMSVN
  TVIPPGEKVVNSDISCHYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVEHPV
  DVAFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPEEAN
  IPIPVKSDMVMIHGHHKETENKEKSALIQQPKQGEEEAFEQGDFYSLLSKLLGEREDVVHVHKYNPTEKT
  ESGSDLVAEIANVVQKKDLGRSDAREGAEHEEGGNAILVRDRIHKFHRLESTLRPAESRALSFQQPGEGP
  WEPELAGDFHVEEALEWPGVYLLPGQVSGVALDSKNNLVIFHRGDHVWDGNSFDSKFVYQQRGLGPIEED
  TILVIDPNKAEILQSSGKNLFYLPHGLSIDTDGNYWVTDVALHQVFKLEPRSKEGPLLVLGRSMQPGSDQ
  NHFCQPTDVAVEPSTGAVFVSDGYCNSRIVQFSPSGKFITQWGEESSGSSPKPGQFSVPHSLALVPHLNQ
  LCVADRENGRIQCFKTDTKEFVREIKHASFGRNVFAISYIPGFLFAVNGKPYFGDQEPVQGFVMNFSSGE
  IIDVFKPVRKHFDMPHDIVASEDGTVYIGDAHTNTVWKFTLTESRLEVEHRSVKKAGIEVPEIKAEAVVE
  PKVKNKPTSSELQKMQEKKKLIKDPGSGVPVVLITTLLVIPVVVLLAIAMFIRWKKSRAFGDHDRKLESS
  SGRVLGRLRGKGSSGLNLGNFFASRKGYSRKGFDRVSTEGSDQEKDEDDGSESEEEYSAPLPTPAPSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 109.3 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_038654
• Locus ID: 18484
• UniProt ID: P97467, F8VQA4, A1L331
• Cytogenetics: 1 47.76 cM
• Refseq Size: 4149
• Refseq ORF: 2934
• Synonyms: PHM
• Summary: Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides (By similarity). Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (By similarity). The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (By similarity). Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (Probable).[UniProtKB/Swiss-Prot Function]