PI 3 Kinase p85 alpha (PIK3R1) Human Gene Knockout Kit (CRISPR)

CAT#: KN210544LP

PIK3R1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: PI 3 Kinase p85 alpha
• Locus ID: 5295
• Components:

  KN210544G1, PI 3 Kinase p85 alpha gRNA vector 1 in pCas-Guide CRISPR vector

  KN210544G2, PI 3 Kinase p85 alpha gRNA vector 2 in pCas-Guide CRISPR vector

  KN210544LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001242466, NM_181504, NM_181523, NM_181524
• UniProt ID: P27986
• Synonyms: AGM7; GRB1; IMD36; p85; p85-ALPHA
• Summary: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]