Human PI 3 Kinase p85 alpha (PIK3R1) activation kit by CRISPRa

CAT#: GA103560

PIK3R1 CRISPRa kit - CRISPR gene activation of human phosphoinositide-3-kinase regulatory subunit 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: PIK3R1
• Locus ID: 5295
• Kit Components:

  GA103560G1, PI 3 Kinase p85 alpha gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103560G2, PI 3 Kinase p85 alpha gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103560G3, PI 3 Kinase p85 alpha gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001242466, NM_181504, NM_181523, NM_181524
• UniProt ID: P27986
• Synonyms: AGM7; GRB1; IMD36; p85; p85-ALPHA
• Summary: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]