Integrin beta 1 (ITGB1) Human Gene Knockout Kit (CRISPR)

CAT#: KN203818

ITGB1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Integrin beta 1
• Locus ID: 3688
• Components:

  KN203818G1, Integrin beta 1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGCTGTGTGTTTGCTCAAAC

  KN203818G2, Integrin beta 1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTACAACCAATTTTCTGGAT

  KN203818D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CTGGCAGAAG TTGATTTTCT TAAAAAAGAG AGATAATAGG AAAACAAGCT CAAAAATCTA TTTGGGGCCT TATGCAGGAT GTTGGCCTTA GCCTGAAGAA ATAAAAACTG ATGAAGCGCA TTGTTAGCAG AGGGATGACT TGATCAGGTT TTTGAAAACG AATGTTCAGC ATTGAGGTAA CTGAGGCATC TGGATATGCT GGTCTGGGCC CCTGGGAGCC GCAGGAGATA ATGGAGATAG AGATTCTATG GGCTGAGACC ATTGGTAGGT TTTATGGTGT TGAGGAATAC TTAACACTGA TTTTTTTTTT GAGCGCTGTT AATTAAATGT TAAAATATAA TCGGTTTGCT TTAGAGAAAG GCACGAATGG ATTCTGGGGC TCTGGTATGG AAGTTATTTT CGCAGTGTAG GTGCAAGGTG AGGGAACCAG ATAGGGAGGG GAGATGGGCG AGGGTCTGTG GAGAGGGGCT GGAGTGAAAT GGTTTGAGAG CCATCATGTT TATAAAAGCA ATAGAAGGTA CGGTGGGGTA ATACTTTCTA TTTAATGCAT TTTTCATTTT ATTTCTTTAT AACTAATATA AGGCACATTT TACATTTCAG TGAGAACCTA AGTCATAATC ATGCTGCTCC TTCTGGAAGC CCTGCAATTG TTCCTCTTAC TCAGAGAAAG GTCAAGTTCC TTCATGGTCA GGCTCCCCTC ACCCCTGCCA CCTGGGATGA TGGGAGGCAA GTTGGCTGGT GAAGTACTAA AATGCTAGGG ATTGAACACA CTGCAAGAAG ATCTTTTGAG AACATGAGAG TGTGTAAGTG AACAGAAGGC AAAAGCAAGT ATATTTAGAA GCAAATGAAA TAAATGTGTA GAATGGAGGA TTATGAACTG TCCATTATTA TCTGCAAAGA CTTTCTAGAA GACATTCTCA CTCTTTGATA AAAAAGTCTC ACAAAATGTT GGCATCATCT AGGAAAGCAG TGAAAATAAA GCTGAAAATG TATTTAACCC TTCTATAAAG ATGTGGCTCC ACATCTGGAA TAATGGATAC GCAGTTCTGT CCTCTGAATC TTGAAAAACT ACACTGCATA GAGCAGTTCG ATATCAGGAT TGGAAGGACT TACTACAAGT AGTAAAAATG AAGCAGGAGA CCGTAAAAGA AAGTATTGAT AAGTTAGTTA CATAAAATCA GTAATAACAA AAACATTCTG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_002211, NM_033666, NM_033667, NM_033668, NM_033669, NM_133376
• UniProt ID: P05556
• Synonyms: CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA; VLAB
• Summary: Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]