HIF-1 alpha (HIF1A) Human Gene Knockout Kit (CRISPR)

CAT#: KN202461LP

HIF1A - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: HIF-1 alpha
• Locus ID: 3091
• Components:

  KN202461G1, HIF-1 alpha gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TTTCTTGTCGTTCGCGCCGC

  KN202461G2, HIF-1 alpha gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGGCGGCGGGCCGAGGGAAT

  KN202461LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  ATCTCCTTTC TCTTTCCTCC GCCGCTAAAC ACAGACGAGC ACGTGAGCGT CGCAGCCCGT CCCAGCTGTG CCTCAGCTGA CCGCCTCCTG ATTGGCTGAG AGCGGCGTGG GCTGGGGTGG GGACTTGCCG CCTGCGTCGC TCGCCATTGG ATCTCGAGGA ACCCGCCTCC ACCTCAGGTG AGGCGGGCTT GCGGGAGCGC GCGCCGGCCT GGGCAGGCGA GCGGGCGCGC TCCCGCCCCC TCTCCCCTCC CCGCGCGCCC GAGCGCGCCT CCGCCCTTGC CCGCCCCCTG ACGCTGCCTC AGCTCCTCAG TGCACAGTGC TGCCTCGTCT GAGGGGACAG GAGGATCACC CTCTTCGTCG CTTCGGCCAG TGTGTCGGGC TGGGCCCTGA CAAGCCACCT GAGGAGAGGC TCGGAGCCGG GCCCGGACCC CGGCGATTGC CGCCCGCTTC TCTCTAGTCT CACGAGGGGT TTCCCGCCTC GCACCCCCAC CTCTGGACTT GCCTTTCCTT CTCTTCTCCG CGTGTGGAGG GAGCCAGCGC TTAGGCCGGA GCGAGCCTGG GGGCCGCCCG CCGTGAAGAC ATCGCGGGGA CCGATTCACC TCGGCGTTAA TGGGATTGGG GGGGGCAGCC TTTTTGTTTC TGCTGCTGCT CCCCTCCCCT CTCTTCCCCC AACCTCGCCG GCCGGGCTCC CCCGCTGTCC ACGTCGCCAT CTTGTCGTGG GGGGTGGGAG ACGCCTCGAA AGTGCTTTCA GGGGCCGGGG TCTGAGCCCT GCTTGCCCTC CCCGCCGGCC GTGGGGGCCT CGCGCCGCCC ACCTACCCGC CTCAAAAACC CAGCCTGCTC TGTGGCCCCA TCCGGAGGGG ACTTTACCCA GCCTGAAAAC CCCGGGAAGA GAAATGAGCT GCAGCTCGGT AGCCGCGGTT TGCACCCGGA GCTTCCGCTC CTTCCCGCCC CCATCCTCTC CAGTTCCATT GAAAACTCGG CCCTGGGGCG GACCCTGCAC GCTGGTCCTG GCTTTCCAGT GGACTTGGGG CCTTGAGTTC CCGACTGAGG GACTCGCGTG GTCGGATGCG ATCTTGTCCT GTAGTTGTCC AGCCGTCGCG GGTGTCTTTG CCTTTGTGCA TTAGGGATTT GCCGCGATGG CCTTAAGATG CGAACTTTTT AGTTTGCACG TGCAGGTTTT GTTTCGTTTT AATCGCCTTG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001243084, NM_001530, NM_181054
• UniProt ID: Q16665
• Synonyms: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
• Summary: This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]