Mouse Rassf1 activation kit by CRISPRa

CAT#: GA205890

Rassf1 CRISPRa kit - CRISPR gene activation of mouse Ras association (RalGDS/AF-6) domain family member 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Rassf1
• Locus ID: 56289
• Kit Components:

  GA205890G1, Rassf1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA205890G2, Rassf1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA205890G3, Rassf1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001243748, NM_019713
• UniProt ID: Q99MK9
• Synonyms: 123F2; AA536941; AU044980; NORE2A; Rassf1A; Rassf1B; Rassf1C; RDA32; REH3P21
• Summary: Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage (By similarity).[UniProtKB/Swiss-Prot Function]