Mouse Prkacb activation kit by CRISPRa

CAT#: GA203240

Prkacb CRISPRa kit - CRISPR gene activation of mouse protein kinase, cAMP dependent, catalytic, beta

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Prkacb
• Locus ID: 18749
• Kit Components:

  GA203240G1, Prkacb gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA203240G2, Prkacb gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA203240G3, Prkacb gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001164198, NM_001164199, NM_001164200, NM_011100
• UniProt ID: P68181
• Synonyms: CbPKA; Pkacb
• Summary: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed:9368018). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA (By similarity).[UniProtKB/Swiss-Prot Function]